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Core Analytical Laboratory

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University at Buffalo, the State University of New York School of Pharmacy and Pharmaceutical Sciences

LCMSMS

Buprenorphine and Norbuprenorphine

Protease Inhibitor

Cortisol, Corticosteriods, Mycophenolic Acid and Mycophenolic Acid Glucuronide

Midazolam, Omeprazole and their Hydroxy Metabolites

Doxorubicin, Doxorubicinol, and Cyclophosphamide

Naltrexone and Naltrexol

Tenofovir

Naltrexone and Naltrexol

Simultaneous LCMSMS Method for Naltrexone and Naltrexol
Analyte	Lower Limit of Quantitation (mg/L)	Acceptable Anticoagulants	Required PlasmaVolume	CV% low QC
Naltrexone	0.005	Heparinized Plasma (Na)	Minimal 1 mL*	11
Naltrexol	0.0005	Heparinized Plasma (Na)	Minimal 1 mL*	7

Samples are prepared by liquid-liquid extraction prior to chromatography. Internal standard (naloxone) is added to 0.5 mL of sample mixed 1:1 acetonitrile and ethyl acetate for twenty minutes. The samples are then centrifuged and the organic layer evaporated to dryness. The reconstituted samples are centrifuged prior to analysis. Ten percent of the reconstituted samples are injected in the LCMSMS system.

Samples are analyzed on an Agilent HPLC system coupled to a Sciex API3000 triple quadupole mass analyzer used in the positive MRM mode. A gradient chromatography method separates the compounds from other components on a Waters Symmetry� C18 column.