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Core Analytical Laboratory

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University at Buffalo, the State University of New York School of Pharmacy and Pharmaceutical Sciences

LCMSMS

Buprenorphine and Norbuprenorphine

Protease Inhibitor

Cortisol, Corticosteriods, Mycophenolic Acid and Mycophenolic Acid Glucuronide

Midazolam, Omeprazole and their Hydroxy Metabolites

Doxorubicin, Doxorubicinol, and Cyclophosphamide

Naltrexone and Naltrexol

Tenofovir

LCMSMS Method for Tenofovir

Analyte

Lower Limit of Quantitation

(mg/L)

Acceptable Anticoagulants

Required Plasma Volume

CV% low QC

Tenofovir

0.010

EDTA or Heparinized Plasma (Na)

Minimal 0.7 mL*

This method was modified and applied in our laboratory Based on the method of Delahunty, et al (see reference). Internal standard (lamivudine) is added to 0.3 mL of sample prior to sample preparation. Samples are prepared by protein precipitation with trifluroacetic acid, pH adjustment and dilution prior to chromatography. An aliquot of the final sample preparation is injected in the LCMSMS system.

Samples are analyzed on an Agilent HPLC system coupled to a Sciex API3000 triple quadupole mass analyzer used in the positive MRM mode. An isocratic chromatography method separates the compounds from other components on a Phenomenex Synergi 4u Polar –RP 80A, 2.0 by 150 mm protected by a like guard column.

Reference: Delahunty T, Bushman L, Fletcher CV, Sensitive assay for determining plasma tenofovir concentrations by LC/MS/MS.

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jan 2;830(1):6-12.